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Further analysis reveals that the ratio of amino, carboxyl, and imidazolyl coordinated with Cu 2+ is close to 1:1:2 according to the ratio of peak area (Fig

In comparison, rhGPx3 mutant seems more likely to catalyze the reaction just like GPx4, which is the only monomeric member among the GPx family, due to the fact that the mutant exists as a monomer in its native state

During this process, we freeze the peptide and expose the frozen compound to low pressure, which safely removes the moisture through sublimation

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12