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Ziegler TR, Mantell MP, Chow JC, Rombeau JL, Smith RJ (1996) Gut adaptation and the insulin-like growth factor system: regulation by glutamine and IGF-I administration

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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader

Table 3), indicating that in this asthma induction model, there is an exacerbated formation of NO , which consequently reacts with the superoxide anion O 2 to form ONOO , responsible for the hyperresponsiveness of asthmatic airways (Sadeghi-Hashjin et al., 1998)