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After washing in phosphate-buffered saline (PBS, pH 7.4), they were fixed in 1% osmium (18456, TED PELLA INC, California, USA) after 12-h gradient ethanol dehydration, and then respectively in epoxy propane (M25514, Myrell, Shanghai, China), 1:1 prepared epoxy propane and epoxy resin (18042, TED PELLA INC, California, USA) were soaked for 30 min and 1.5 h, then embedded in epoxy resin, and baked at 40C and 60C ovens for 12 and 48 h, respectively

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