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Utilizing Light Cycler 480, SYBR Premix Ex Taq TM II, and gene-specific primer pairs, quantitative real-time PCR was carried out using generated cDNAs as templates (Roche)

1 Introduction 1.1 Diabetic neuropathy Diabetic neuropathy (DN) is a significant complication of diabetes mellitus, characterized by nerve damage resulting from prolonged hyperglycemia