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Patient-centered outcomes Participants were asked to complete the following questionnaires on D3, D7, and D14

The homogenate was centrifuged at 10,000 g for 15 min and aliquots of supernatant were separated and used for biochemical estimation

Tico Times

Immunoassay Tear fluid was extracted from half of the remaining strip by agitating small cut pieces of these strips in 100 L PBS, Tween20 0.05% and cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) at 4 C for 1.5 h 28