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This creates a 10-day gap between doses, which is longer than ideal but much safer than cutting it close

By layering dissociated spinal cord cells over a four-step discontinuous gradient of 4.5%, 6%, 7.5% and 10.5% (w/v) iodixanol (OptiPrep initially diluted to 30% iodixanol with 0.15 M NaCl, 10 mM MOPS, pH 7.4 and then further diluted with Hanks Balanced Salt Solution) and centrifuging at 1900 rpm for 15 min., debris remained at the top interface, neurons banded at the three lower interfaces and inflammatory cells and glial cells pelleted [22,23]

Chun HJ, Kim YS, Kim BK, Kim EH, Kim JH, Do BR, Hwang SJ, Hwang JY, Lee YK: Transplantation of human adipose-derived stem cells in a rabbit model of traumatic degeneration of lumbar discs

You might experience a heavy sensation in the affected area, and pain may worsen with strenuous activity