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The following primary antibodies were used for western blotting in this study: rabbit anti-GFP (1:3000, TP401, Torrey Pines Biolabs), mouse anti-actin (1:5000, 66009-1-lg, Proteintech), mouse anti-GAPDH (1:2000, 60004-1-1 g, Proteintech), rabbit anti-CTSD (1:1000, 21327-1-AP, Proteintech), rabbit anti-LC3 (1:2000, 14600-1-AP, Proteintech), rabbit anti-HRD1 (1:4000, 13473-1-AP, Proteintech), rabbit anti-Golgin160 (1:1000, 21193-1-AP, Proteintech), mouse anti-HA (1:500, 16B12, BioLegend), mouse anti-CTSC (1:500, D-6, Santa Cruz Biotechnology), mouse anti-VPS35 (1:500, sc-374372, Santa Cruz Biotechnology), rabbit anti-LAPTM4A (1:1000, HPA068554-1, Millipore-Sigma), rabbit anti-LYSET (1:1000, HPA-48559, Millipore-Sigma), mouse anti-V5 (1:3000, 46-0705, Invitrogen), rabbit anti--COP (PA5- 21484, Invitrogen), rabbit anti-VPS26 (1:1000, ab23892, Abcam), rabbit anti-GOLPH3 (1:2000, AB236296, Abcam), rabbit anti--COP (ab2899, Abcam), anti-GCN4 [IPI-C11L34] (1:1000, 218104-rAb, Addgene), mouse anti-LAMP2 (1:1000 H4B4-c, DSHB)

Apr 2014;20(3):278-84

Across the six published trials, the most common adverse events were headache, mild gastrointestinal effects (diarrhea, flatulence, nausea), nasopharyngitis, fatigue, dizziness, and mild injection-site reactions

EMD Millipore, Billerica, MA)