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Using liquid chromatography coupled to mass spectrometry (LCMS), we detected picogram quantities of erucamide in peripheral retinal tissue from postmortem human donor eyes ( n = 6, age range 6285 years) from Lions NSW Eye Bank, Sydney Local Health District Ethics Review Committee (2020/ETH03339), suggesting that it is ubiquitously expressed in the retina across multiple species (Extended Data Fig

The principle behind the assay is that oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm

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