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The NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used to generate the sequencing libraries, which were sequenced on an Illumina Hiseq platform (Illumina, San Diego, CA, USA) to generate paired-end reads

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It is important to note that as a safety precaution, the IAOMT does not recommend amalgam filling removal for women who are pregnant or breast-feeding and that the IAOMT does not recommend that dental personnel who are pregnant or breast-feeding conduct work that disrupts amalgam fillings (including their removal)