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10.1016/j.nbd.2016.05.011 13 DuJ

The prepared supernatants reacted with Assay Buffer and Iron Reducer for 30 min

Typically, this temperature range should be between 20C and 25C (68F and 77F)

( f ) In vivo ubiquitination assay: HA-ubiquitin (HA-Ub) was transfected to control (Ctrl) and FEM1B KO cells stably expressing PLD6-FLAG, followed by IP with anti-FLAG beads