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While p53 modulators, including FOXO4-DRI, MDM2 inhibitors, and USP7 inhibitors have shown senolytic effects by selectively eliminating senescent cells or promoting their apoptosis in the laboratory, translating these findings to the clinic have so far been unsuccessful

However, most of the experimental tests have been conducted in animal models and in vitro experiments, and only a few clinical trials have been conducted

Expression and purification of recombinant methyltransferase proteins NSD1(SET) (amino acids 18532093, NCBI sequence: NC_000005.10), NSD2(SET) (amino acids 9581365, NCBI sequence: NC_000004.12), NSD3(SET) (amino acids 10211320, NCBI sequence: NC_000008.11), ASH1L(SET) (amino acids 19802564, NCBI sequence: NC_000001.11), SETD2(SET) (amino acids 13232564, NCBI sequence: NC_000003.12) and SUV39H1(SET) (amino acids 82412, NCBI sequence: NC_000023.11) were cloned into pGEX-6P-1