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99,100,101 A study analysing 21 brain metastases and their matched primary breast cancer showed that BCBM tends to have high genomic-aberration-based-homologous recombination deficiency (HRD) scores compared with their primary tumour counterparts, which indicates that brain metastases might be more sensitive to PARP inhibitors than their corresponding primary tumours

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Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on molecular structure, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times

These precision editing modalities represent significant advances over conventional CRISPR-Cas9 nucleases, particularly in post-mitotic hepatocytes where HDR is inherently inefficient