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2.2.2 Thermal stress of myocytes Myocytes were seeded at a density of 110 6 per well in a 24-well plate, and subjected to high-temperature culture at 37C for 2, 4, 6, 12, and 24 hours

The most common types of medication to increase the appearance of dark patches are non-steroidal anti-inflammatory drugs (NSAIDs), tetracyclines, and psychotropic drugs

The commonly used method is high-performance liquid chromatography, supplemented by other validation methods, as detailed below

These cells show an increase in telomeric sister chromatid exchange (T-SCE) and rapid telomere elongation using ALT recombination mechanisms (Murga et al 2007)