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Some people find that proper injection technique eliminates site reactions entirely

By suppressing cytotoxic CD8+ T-cells, expanding regulatory T cells, and promoting myeloid-derived suppressor cells (MDSCs), VEGF facilitates tumor immune evasion ( The VEGF signaling axis is heterogeneous, comprising multiple ligands (VEGF-A, -B, -C, -D, and placental growth factor) and receptors (VEGFR-1, -2, -3) as well as co-receptors such as neuropilins ( + T cells, expanding regulatory T cells, and increasing myeloid-derived suppressor cells (MDSCs), VEGF promotes tumor immune evasionmechanisms corroborated in translational studies and consistent with the synergy observed when VEGF blockade is combined with immune checkpoint inhibitors (Figure 1)

Are there any references to studies that show these effects really take place

Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)