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Nuclease degradation, endosomal trapping, rapid and extensive renal excretion, and limited cellular uptake, are some of the limitations that siRNAs face to exert their therapeutic effect ( Over-expression of epidermal growth factor receptor (EGFR) mainly in triple negative breast cancer is known to promote tumor survival and suppress apoptosis ( We employed Gold nanoparticles (AuNPs) for efficient packaging and delivery of siRNA against EGFR, as AuNPs are good candidates for nucleic acid delivery due to their distinctive features including simple synthesis enabling the control of size and shape, Surface Plasmon Resonance (SPR), easy functionalization, and biocompatibility ( We chose a chitosan derivative to coat the AuNPs

These mechanisms together may explain why B12 has been studied in conditions such as diabetic neuropathy, nerve injury, and radicular pain syndromes similar to sciatica

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