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the cofactor required by aminotransferases) and one of the following pairs of substrates (5 mM each): pyruvate + glutamate for alanine aminotransferase (ALT) assays, alanine + -ketoglutarate for reverse ALT assays, oxaloacetate + glutamate for aspartate aminotransferase (AST) assays, aspartate + -ketoglutarate for reverse AST assays, and pyruvate plus one of each of the amino acids listed in Fig