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In that study, the authors used young adult (8-10 weeks old) male C57BL/6 mice and performed a dosage screen (from 0.1% to 0.6%) by examining the demyelination in the corpus callosum of cerebral white matter, a brain region where the extent of demyelination can be scored more easily and consistently than the previously described superior cerebellar peduncles with LFB-PAS staining (Skripuletz et al., 2011)