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The random and site-saturation libraries contained a similar number of mutations on average (random = 2.79, site-saturation = 2.99), therefore, the lower fold induction in the site-saturation library may be attributed to less conservative mutations accessible via the NNS codon compared with single-nucleotide substitutions (Supplementary Fig

Validation of target genes in secondary screenings A curated list of target genes (Supplementary Dataset 3), which emerged from the shRNA screen and subsequent GO analysis, was used for further validation with siRNAs selected from the Human Druggable Genome siRNA Library (Dharmacon)

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Nayan Patel: And so that's, there's, there's my technology