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Immediate emergency care is warranted if abdominal pain reaches 7/10 severity, especially when combined with confusion, easy bruising, or swelling in the legs and abdomen indicating potential liver failure

Indeed, HA-tagged NEDD8 or ubiquitin, but not SUMO2 or SUMO3, immunoprecipitated modified high-molecular weight IL-1 when expressed in 293T cells (Supplementary Fig

Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)