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UBE2D2 and Ub were isolated using cation-exchange chromatography and eluted with either 50 mM HEPES pH 7, 250 mM NaCl, 5 mM DTT or 25 mM sodium acetate pH 4.5, 250 mM NaCl, respectively

Selection criteria for the eta-analysis Inclusion criteria were studies (1) including healthy participants, (2) utilising 1 H-MRS to measure GSH levels in specified brain regions, (3) giving mean and SD for each group, (4) patients with depression only and not bipolar affective disorder in accordance with criteria specified in the Diagnostic and Statistical Manual for Mental Disorders (DSM) or the International Classification of Diseases (ICD-11), (5) information provided on sex of patients

It is also worth noting that -lipoic acid, a metabolite in the cuproptosis pathway, has shown promising results in both cell-based and animal models of Wilsons disease

Biochemistry 55, 42864294