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Total alkaloid content was ascertained at 5.03% by means of high-performance liquid chromatography (HPLC) according to methods described elsewhere [22]

After centrifugation at 3000 g for 10 min, the supernatants were evaporated in a vacuum centrifugal evaporator EC-57CS (Sakuma, Tokyo, Japan) and dissolved in 1% (v/v) acetic acid

Learn More About Vitamin B-12 Contraindications Tell your provider about your other medicines, especially: chloramphenicol, colchicine, antibiotics, metformin, or medicines that reduce stomach acid, such as cimetidine (Tagamet), omeprazole (Prilosec), lansoprazole (Prevacid), esomeprazole (Nexium), and ranitidine (Zantac)