After blocking with 5% non-fat milk (BioRad) or bovine serum albumin (BSA) (Sigma-Aldrich), in tris-buffered saline, 0.1% Tween 20 (TBST), membranes were incubated with primary antibodies at 4C overnight
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It has also been shown that intergenic HBS1L-MYB SNPs are not correlated with HU treatment [53], [60]
