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Description

The aggregates were collected as described above and resuspended at 3.5 ml/million cells in Knockout DMEM (Life Technologies), 20% (vol/vol) Knockout Serum Replacement (Life Technologies), 1% dimethyl sulfoxide (Sigma-Aldrich), nonessential amino acids (NEAALife Technologies), 2-mercaptoethanol (Life Technologies) and Glutamax (Life Technologies) and incubated for 5 days, with a medium change every 48 hours

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b) Monoclonal antibodies directed against HGF and Met

However, DOX-treated group displayed glomeruli distortion, filtration space obliterated disappear, tubules focal atrophy necrosis and exfoliation and vascular congestion