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At 12 hpi, cells were lysed by resuspending in 3 mL of mild lysis buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 2 mM MgCl 2 1 mM EDTA, 1 mM DDT and 0.5 % Igepal) and incubated on ice for 10 min, followed by vortexing for 30 s

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Incidence and Mortality Estimated new cases and deaths from thyroid cancer in the United States in 2025:[2] New cases: 44,020

The studies and documents that met the criteria were assessed, identified, and graded by all authors independently, and any discrepancies were resolved through discussion and consensus