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Kurokawa et al., 2014a, b)

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Assessment of liver fibrosis with transient elastography and FibroTest in patients treated with methotrexate for chronic inflammatory diseases: a case-control study

Captured mouse IgG was detected using an AP-conjugate anti-mouse IgG (Fc-specific from goat (Sigma-Aldrich, A9316) (diluted 1:5000 in PBS/T/S) and visualized by addition of AP-substrate (10 ug/mL in diethanolamine buffer) (Sigma-Aldrich)