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Cells were cultured in DMEM (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham), 100 g/mL pyruvate, 50 g/mL uridine, and 100 U/mL penicillin-streptomycin at 37 C with 5% CO

Afterward, the deprotection solution was removed, and the discs were solubilized overnight (ON) at rt, while shaking, using a solvation mixture containing 88.5% TFA, 4% trifluoromethanesulfonic acid (TFMSA), 5% H 2 O and 2.5% TIPS (250 L/well)

First, an enzyme called alcohol dehydrogenase (ADH) converts alcohol into acetaldehyde, a toxic compound

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