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The T-box protein Brachyury, a transcription factor, promotes the resistance of breast cancer cells to tamoxifen by inhibiting SIRT1 (Li et al., 2016)

DOI: Published: Publisher Name: Springer, Berlin, Heidelberg Print ISBN: 978-3-662-47397-9 Online ISBN: 978-3-662-47398-6 eBook Packages: MedicineReference Module Medicine

Add the elution buffer to the column, cap both ends, and incubate for a few minutes before uncapping and dripping your elution out of the bottom (Figure 4)