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Description

Briefly, total RNA from cells was isolated using a Pure link RNA Mini kit (Life Technologies, USA), according to the manufacturers protocols

Buffer 1 SGF without enzyme Buffer 2 SGF with 0.32% pepsin Buffer 4 SIF with 1% pancreatin SIF was prepared freshly with 8.7 mM NaOH, 28.65 mM NaH2P04, 105.85 mM NaCl, with a final pH of 6.8