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Description

Immunolabeling visualization was performed using the Vectastain ABC and DAB kits (Vector Laboratories, Inc., Burlingame, CA)

Growth hormone secretagogues elevate GH and subsequently can affect glucose

A limitation of the study was that all these sequences were not confirmed by transcriptional analysis or by sequencing of the corresponding equine cDNA

Future studies should validate our findings in genetic models to determine whether the observed gut-brain interactions are generalizable across ASD subtypes