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The changes presented here directly influenced selenoprotein expression and host antioxidant metabolism

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GST pulldown assay Differentiated 3T3-L1 cells were lysed in immunoprecipitation (IP) lysis buffer (20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, and 1 mM EGTA, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na 3 VO 4 , 1 mM PMSF, and 1X PIC)

Additionally, a loss of thioredoxins or thioredoxin reductase results in a threefold increase in the concentration of reduced GSH