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In another study they described a similar GSH reduction in red blood cells of sALS patients treated with insulin-like growth factor 1 (IGF-1), but probably only as a secondary cytotoxic effect due to the treatment with IGF-1 [103]

[60] treated THP-1 cells with the SDH inhibitor atpenin A5 (AA5), which provoked HIF-1 stabilization and concomitated HIF-1 target gene activation, with the macrophage phenotype shifting to classical cell activation

Protein transfer was confirmed using reversible protein stain (Memcode, Life Technologies)

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