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The next day, cells were transfected with 25 nM of small interfering RNA (siRNA)to GCLM (Dharmacon L-011670-01-0005) or a non-targeting negative control (ON-TARGETplus Non-targeting Control Pool, Dharmacon #D-001810-10-05) using 50 nM of DharmaFECT4 (Horizon Discovery, T-2005-01) as a transfection reagent