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To analyse the effect of CO, protein samples were prepared in a deaerated solution and treated with dithionite at 5 mmol l 1 and/or CO gas

This anti-inflammatory action is mediated through several signalling pathways, including the inhibition of the nuclear factor kappa B (NF-B) pathway, contributing to the reduction of liver inflammation associated with NAFLD

A subset of animals were euthanized on D3 and D6 and their lungs were removed and homogenized in cold PBS to minimize degradation before total RNA extraction