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Analysis of pre- and post-vaccination whole blood transcriptomes As IFNG gene expression analysis yielded some encouraging results but retained ambiguity due to the pre-existing immunity to latent infection, we further investigated whole transcriptome changes in the two stage II elephants that had received the full vaccine doses at extended intervals

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RNA analysis Tissue samples were crushed on dry ice and homogenized using a bead Mill (VWR)

Cell 67 : 879888 Seymour CB, Mothersill C, Alper T (1986) High yields of lethal mutations in somatic cells that survive ionizing radiation