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2.1.1.1 All- trans retinoic acid (ATRA) CD38 expression can be modulated both in vitro and in vivo through the nuclear retinoic acid receptor ( in vivo use, has proven effective in enhancing CD38 levels in several ex vivo myeloma cell preparations, leading to improved efficacy of therapeutic antibodies ( in vivo efficacy in combination with Daratumumab was called into question in a clinical trial involving relapsed/refractory (RR) MM patients

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For NEDD8 , it was cloned with N-terminal HA tag

Because of these complexities, and significant uncertainty in defining metalloproteomes (Cvetkovic et al., 2010), it is often challenging to define the precise set of metal ions that are required for cells