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The filtrate was diluted 200 or 1,000 times with 0.1% formic acid in Milli-Q (MQ) water and then applied to an LCMS-8040 system (Shimadzu Co.) to quantify felinine and its related compounds, 3-methylbutanol glutathione (MBG), 3-methylbutanol cysteinyl-glycine (MBCG), and N -acetylfelinine, based on a previous report ( m/z 208.2 122.0 [collision energy (CE) 12 eV] for felinine, m/z 265.1 178.9 (CE 11 eV) for MBCG, m/z 394.2 179.0 (CE 17 eV) for MBG, and m/z 250.1 121.9 for N -acetylfelinine

Testing is conducted using RP-HPLC with UV detection under controlled laboratory conditions

Trace element enzymes are involved in many biological and chemical processes

The consequent derailment of the cellular behavior can thus lead towards apoptosis (a natural damage-limiting self-digestion of critically damaged cells, [85]), necrosis (an auto-sustaining, propagating tissue damage) and towards neoplastic transformation (an uncontrolled, invasive cellular growth)