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Rahbar MH, Samms-Vaughan M, Lee M, Zhang J, Hessabi M, Bressler J, Bach MA, Grove ML, Shakespeare-Pellington S, Beecher C, McLaughlin W, Loveland KA (2020) Interaction between a mixture of heavy metals (lead, mercury, arsenic, cadmium, manganese, aluminum) and GSTP1, GSTT1, and GSTM1 in relation to autism spectrum disorder

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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)