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Expression of the cancer stem cell markers CD44 and CD133 in colorectal cancer: An immunohistochemical staining analysis

10.1126/sciadv.1601167 381 YinH

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A cDNA library was synthesized using BD Biosciences Clonetech SMART cDNA library Construction kit (User Manual PT3000-1 [PR15738]) and cloned between the SfiIA and Sfi IB sites of plasmid pTriplEx2 (Clonetech)