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Liver homogenates were prepared under the same conditions used for the citrate synthase assay and then diluted 4:1 in Laemmli buffer (100 mM Tris-HCl, 2% SDS, 10% glycerol and 0.1% bromophenol blue) for NRF-1 determinations

The method according to claim 1 or 2, the use according to any one of claims 3-6, or the composition according to claim 7 or 8, wherein the Autism Spectrum Disorder (ASD) comprises syndromic autism, autism of known etiology, fragile X syndrome, PTEN macrocephaly syndrome, RETT syndrome, tuberous sclerosis complex, Timothy syndrome, or Joubert syndrome

(2013) Dietary intake and plasma levels of choline and betaine in children with autism spectrum disorders, Autism research and treatment, Vol

Bailey SJ, Blackwell JR, Lord T, Vanhatalo A, Winyard PG, Jones AM