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For H 2 O 2 resistance, flies were transferred onto medium containing 5% v/v H 2 O 2 (H1009, Sigma), 5% w/v sucrose, 1.5% w/v agar

By binding to specific DNA sequences and protecting promoter regions from methylation, Testagen helps to maintain transcriptional activity in genes involved in hormone synthesis, with potential implications for sustaining optimal testosterone production under physiological stress or aging conditions 1-3 .