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Osteopontin regulates human glioma cell invasiveness and tumor growth in mice

Warawa JM, Long D, Rosenke R, et al

Cell pellets were harvested by centrifuging at 4000 rpm, 4 and stored at -20 C

Diseases associated with RAGE activation include inflammatory diseases, rheumatic or autoimmune diseases, infectious diseases, diabetes, metabolic syndrome and its complications, obesity, insulin resistance, hypertension, atherosclerosis, neurological diseases such as Alzheimers disease, cardiovascular diseases, pulmonary diseases such as chronic obstructive pulmonary disease (COPD), and cancer.[ref] (Yes, that is pretty much every chronic disease that I can think of and all are associated with aging.) Activation of RAGE (cell membrane receptor) causes an increase in reactive oxygen species as well as an increase in inflammatory cytokines