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At 24, 48, 72, and 96 h after seeding, the cells were trypsinized to obtain single-cell suspensions, and cell numbers were measured using a Vi-CELL XR cell counter (Beckman Coulter, Tokyo, Japan)

In plain terms, while the raw percentage increases varied, the variation could easily be explained by chance rather than a true superiority of one method over another

Eur J Gastroenterol Hepatol 11:11851194 Oksanen J, Simpson G, Blanchet F, Kindt R, Legendre P, Minchin P, OHara R, Solymos P, Stevens M, Szoecs E et al (2025) Vegan:community ecology package