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Trials typically incorporate biomarker endpoints such as ER/ER ratios, aromatase activity, plasma isoflavone or polyphenol concentrations, immune cell phenotyping, cytokine panels, oxidative stress markers and microbiome profile which are used to provide mechanistic read-outs of supplement activity and to inform patient stratification approaches (231)

Cells were cultured at 37 C with 5% CO 2 and subpassaged using a 1-in-4 dilution when a confluency of approximately 80% was reached

The supernatant was used for phenol and flavonoid estimation

At the tissue level, paracrine signals are required to synchronize cell populations