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Per treatment, we administer: Thanks to the moderate dosage per infusion, the risk of side effects and allergic reactions can be reduced

Cells were washed twice with ice-cold HEPES-buffered serum-free media, pH 7.4, and either incubated for 1 h on ice with 500 nM hemeAF546HPX for surface binding or transferred to 37 C for 10 min for endocytosis

Interestingly, several studies showed that cytokines and stress stimuli such as TNF-, interleukins, FAS ligand, heat shock, UV irradiation and also stressors that deplete glutathione such as ROS and 4HNE can activate sphingomyelinase, which leads to increased ceramide production 58,59,60