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Mouse Interleukin 2 ELISA Kit (E-EL-M0042) and MS Mouse interferon gamma ELISA Kit (E-MSEL-M0007) (Elabscience, China) were used to detect the cytokine concentration and the absorbance was measured at OD 450 using a Varioskan LUX microplate reader (Thermo Scientific)

Nevertheless, SSE may remain the most critical parameter affecting accurate quantification, as variables such as individual water uptake, nutrition, sex, age, health, and socio-economic status can affect the biological matrix

The work of Tsuchiya et al., published in Nutrients , highlights how cystine and theanine stabilize metabolic parameters, reduce inflammation markers like IL-6, and minimize energy expenditure post-surgery

Though this approach, based on the analysis of metabolic profiles, resulted useful and revealing, we must acknowledge its limitations