glutathione peroxidase analysis A validated method to assess enzyme activity | Chemical Papers Glutathione Peroxidase-1 in Health and

The peptide sequence (residues 110128) was D-P-V-G-S-P-T-S-G-A-S-L-N-G-T-P-F-K-K-K, with an additional lysine added for charge

For the analysis of gut microbiota, Alpha diversity metrics, including Chao1 and Shannon index, were calculated using the mothur software ( The Wilcoxon rank-sum test was employed to analyze differences in Alpha diversity between groups

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For assay of GPX, crude enzyme protein (100 g equivalent) was incubated in assay mixture containing 100 mM potassium phosphate buffer (pH 7.5), 0.5% H 2 O 2 and 0.5 mM ortho-dianisidine