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GABA is then stored in vesicles waiting for release following neuronal activation [67]

Western blotting Cells were lysed in modified RIPA buffer (50 mM TrisHCl (pH 7.5), 150 mM NaCl, 10 mM -glycerophosphate, 1% NP-40, 0.25% sodium deoxycholate, 10 mM sodium pyrophosphate, 30 mM sodium fluoride, 1 mM EDTA, 1 mM vanadate, 20 mg/ml aprotinin, 20 mg/ml leupeptin, and 1mM phenylmethylsulfonyl fluoride)