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Proteins were detected by chemiluminescence using Immobilon Classico or Immobilon Forte (Merck), Lumi-Light substrate (Roche), or SuperSignal West Femto (Thermo Fisher) on a ChemiDoc system (Bio-Rad), and band intensities were quantified with Image Lab/Fiji

Of notice, glucose is still highly absorbed by SGLT2 in IR states, suggesting that this mechanism is not affected by IR, though it is upregulated by hyperinsulinemia