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Liver health: The liver is responsible for numerous vital functions, including detoxification, metabolism, and synthesis of essential compounds

Protein concentrations were determined by the bicinchoninic acid (BCA) method (Pierce, Rockford, IL), and 20 g of protein were subjected to SDS-PAGE and western blot analysis

For each microtubule segment all but the signal from two adjacent protofilaments was substracted to generate a two protofilament stack, followed by refinement of particle coordinates