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Recommended endpoints: histology, functional assays, angiogenic markers (CD31, VEGF), and blinded scoring to reduce bias

Published research indexing: PubMed record on cagrilintide and semaglutide

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The nanoBRET signal from assay wells was corrected by subtracting the 618 nm/460 nm ratio of cells co-expressing NanoLuc and HaloTag minus the nanoBRET ratio of cells expressing only the NanoLuc in the same experiment